Career & Expertise
My professional experience, academic background, published research, and continuous learning.
Career Path
Experience
Clinical Embryology Intern
Indira IVF and Fertility Center — Gorakhpur, India
Semen analysis, semen preparation, semen freezing & thawing, DFI, dish preparation, oocyte scanning, denudation, embryo freezing & thawing, oocyte freezing & thawing, embryo transfer, ICSI (Narishinge), RI witnessing, HIS.
Assistant Embryologist
Manmohan IVF Center Pvt. Ltd. — Kathmandu, Nepal
Assisted in embryo culture, cryopreservation, and IVF laboratory management.
Medical Lab Technologist
Alka Hospital Pvt. Ltd. (Alka IVF) — Kathmandu, Nepal
Sperm analysis and semen preparation for assisted reproduction procedures.
Biotechnologist
Kirtipur Municipality TU Biotech Corona Lab — Kirtipur, Nepal
Supported development and testing of PCR-based diagnostic assays.
Microbiologist Trainee
Royal SaSa Nepal Pharmaceutical Pvt. Ltd. — Chitwan, Nepal
Microbiological testing on pharmaceutical products and quality control.
Microbiology Lab Intern
Green City Hospital — Basundhara, Kathmandu
Diagnostic testing, sample collection, lab procedures for infectious disease testing.
Academic Background
Education
M.Sc. Clinical Embryology
✓ CompletedSEART (School of Embryology and Assisted Reproduction Technology), Shridhar University — India
Gold Medalist (1st Position) · Highest CGPA · Comprehensive 2-year postgraduate training in Assisted Reproduction Technology
M.Sc. Biotechnology
Central Department of Biotechnology, Tribhuvan University — Kathmandu
Thesis: Evaluation of Contraceptive Properties of Medicinal Plants Dioscorea bulbifera
B.Sc. Microbiology
Tri Chandra Multiple Campus, Tribhuvan University — Kathmandu
Higher Secondary (+2 Science)
Tilottama Campus, HSEB — Nepal
School Leaving Certificate
Everest English Boarding School — Butwal
Academic Work
Research & Projects
A Study of the Association of AMH Levels with AFC, Age and Etiology among Women Undergoing ART Cycles
Investigated the relationship between Antimullerian Hormone (AMH) levels, Antral Follicular Count (AFC), patient age, and underlying etiology in women undergoing assisted reproduction cycles.
Evaluation of Contraceptive Properties of Dioscorea bulbifera by In Vitro Sperm Function and In Vivo Fertilization Assay
Explored the contraceptive potential of the medicinal plant Dioscorea bulbifera through sperm function tests and fertilization assays.
Mouse Acrosome Staining Using Various Methods
Analyzed sperm acrosome integrity using different staining techniques to evaluate acrosomal status and sperm capacitation.
Antibiotic Susceptibility Pattern of Bacteria Isolated from Blood Samples
Studied the antibiotic susceptibility profiles of bacterial isolates from clinical blood samples to inform treatment protocols.
Published Research
Publications
Dioscorea bulbifera tuber extract causes sterility in mice
Investigated contraceptive properties of Dioscorea bulbifera tuber extract — effects on mouse sperm acrosome reaction, viability, and membrane integrity. Confirmed significant herbal contraceptive potential.
Read PaperArtemisia vulgaris Extract Causes Precocious Acrosome Reaction and Viability Loss but Low Rate of Membrane Damage in Mouse Spermatozoa
Demonstrated direct contraceptive effects of Artemisia vulgaris on sperm viability and acrosome reaction in vitro and in vivo.
Read PaperContinuous Learning
Professional Development
Hands-on Training: RI Micromanipulator and ICSI
Cooper Surgical & SEART
Non-Invasive Genetic Sampling of Preimplantation Embryos
International IVF Initiative
"How We Operate" — Online Meeting
International IVF Initiative
"Odyssey: A Journey into Genetic Makeup (Basic to Advanced)"
i-Ceat
Implementing Total Quality Management in ART Centers
Workshop
"Entering a New Epoch of ART in 2024" — Conference
The Leela Hotel, Gurugram, India
Hands-on Training Program
Embryology Academy for Research and Training, Mumbai
Training Assistant: CDBT-TU 2nd Winter School — Experimental Biotechnology
A Teachers' Training Workshop
Volunteer: International Workshop on Advancing Biological and Clinical Research with Flow Cytometry
International Workshop
Symposium: "A Genetic Approach to Blood Disorders"
KCGRL
References
Referees
Abhinav Dudeja
Senior Embryologist, Harley Street Fertility Center; Senior Faculty, Shridhar University; Assistant Professor, IARS
abhinavembryologist@gmail.comDr. Gaurishankar Manandhar
Professor, Central Department of Biotechnology, Tribhuvan University
gaurishankar.manandhar@gmail.com